Nageen Sharma
Craniosacral therapy
Faisalabad, Punjab, Pakistan
Years of experience 3

Total years in practice: 3

Published Date
January 01, 2010
Abstract Authors
Werayut Pothitirat, Mullika Traidej Chomnawang, Wandee Gritsanapan
Abstract Source
Med Princ Pract. 2010;19(4):281-6. Epub 2010 May 26. PMID: 20516704
Abstract Affiliation
Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
Study Type
Research
Conditions
Acne
Therapies
Functional Medicine, Naturopathic Medicine
Reference
Abstract Content
OBJECTIVE:
The aims of this study were to determine the most effective solvent extract of mangosteen, anti-acne- inducing bacterial activity and the amount of alpha-mangostin, a major active component in each mangosteen fruit rind extract, using high-performance liquid chromatography (HPLC). MATERIALS AND
METHODS:
The fruit rinds of mangosteen were extracted with hexane, dichloromethane, ethanol and water. The extracts were tested for antibacterial activity against bacteria that induce acne, including Propionibacterium acnes and Staphylococcus epidermidis. Thin-layer chromatographic autobiography against these bacteria was also performed for each extract, while the alpha-mangostin content was analyzed using a validated HPLC method.
Results:
The dichloromethane extract exhibited the strongest antibacterial effect with minimum inhibitory concentration values for both bacterial species at 3.91 microg/ml, while the minimum bactericidal concentration values against P. acnes and S. epidermidis were 3.91 and 15.63 microg/ml, respectively. Thin-layer chromatographic autobiography indicated that alpha-mangostin was present in all extracts, except the water extract, and is a major active component against both P. acnes and S. epidermidis. Using HPLC, the dichloromethane extract yielded the highest content (46.21% w/w) of alpha-mangostin followed by the ethanol extract (18.03% w/w), the hexane extract (17.21% w/w) and the water extract (0.54% w/w). CONCLUSIONS: Dichloromethane extract exhibited the strongest anti-acne-inducing bacterial effect and this extract yielded the highest amount of alpha-mangostin.
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